Device

Part:BBa_J119395:Design

Designed by: Todd Eckdahl   Group: Eckdahl_Lab   (2016-06-23)


tClone Red containing the RS3 Riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 765
    Illegal AgeI site found at 877
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BsaI sites were used to join the RS3 riboswitch to tClone Red


Source

Wachsmuth et. al. (2013)

References